兰州黄河岸边卵石层大吨位桩基自平衡法试验研究

对兰州深安黄河大桥进行桩基自平衡法试验。在对黄河岸边的岩土层进行桩基试验时,对桩基的极限侧阻力和端阻力标准值的取值提出建议,并在兰州地区进行自平衡法桩基试验时,对桩基侧摩阻力的取值提出建议。     

柏拉图关于城邦卫士的教育思想及其启示

在柏拉图构建的理想国中,以保卫城邦为职业的城邦卫士的工作尤其重要。具有良好品质的好卫士,既是护卫理想城邦所必需,也是培养和造就未来的统治者哲学王的基础。柏拉图认为培养一个好卫士的必由之途是教育,他提出了实施音乐教育和体育教育,以达到内外双修的教育目的,造就美善兼备的理想卫士。柏拉图注重"立人"的教育理念和人才观,对于我们今天仍存在诸多问题的教育事业,有着深刻的启迪和较强的借鉴意义。     

The expansion of GPCR transactivation-dependent signalling to include serine/threonine kinase receptors represents a new cell signalling frontier

G protein-coupled receptor (GPCR) signalling is mediated through transactivation-independent signalling pathways or the transactivation of protein tyrosine kinase receptors and the recently reported activation of the serine/threonine kinase receptors, most notably the transforming growth factor-β receptor family. Since the original observation of GPCR transactivation of protein tyrosine kinase receptors, there has been considerable work on the mechanism of transactivation and several pathways are prominent. These pathways include the “triple membrane bypass” pathway and the generation of reactive oxygen species. The recent recognition of GPCR transactivation of serine/threonine kinase receptors enormously broadens the GPCR signalling paradigm. It may be predicted that the transactivation of serine/threonine kinase receptors would have mechanistic similarities with transactivation of tyrosine kinase pathways; however, initial studies suggest that these two transactivation pathways are mechanistically distinct. Important questions are the relative importance of tyrosine and serine/threonine transactivation pathways, the contribution of transactivation to overall GPCR signalling, mechanisms of transactivation and the range of cell types in which this phenomenon occurs. The ultimate significance of transactivation-dependent signalling remains to be defined but it appears to be prominent and if so will represent a new cell signalling frontier.     

海、陆两用风力发电实验平台设计

在风电场通过现场测试分析研究风力机性能由于受到现场环境的限制,能够获得的研究数据有限,室内模拟实验平台能够有效弥补研究不足.为此提出了一种海、陆两用风力发电实验平台方案.该实验平台由来风装置、风轮、发电机、两段式塔架、漂浮台、波浪产生装置、传感检测和数据采集系统等构成.在实验平台整体方案基础上,进行了结构参数设计,建立了漂浮台受力分析模型并获得了合理的结构参数,以Lab VIEW为开发环境设计了上位机运行状态监控系统.最后,基于构建的实验平台,开展了部分风力机动态特性研究工作,对比分析了模拟陆地风力机和海上漂浮式风力机时塔架振动特性,为进一步深入开展风力机动态特性研究提供了基础.     

基于混沌映射公钥系统的多身份验证机制算法及应用

多身份验证是在两人或两人以上在验证中心的验证下开启一件装备(设备).在一些特殊的领域有一些应用,特别是在一些网络身份验证无法实施的地方有重要应用.比如:在军 事应用方面:远程重型装备投送后多身份验证开启,移动枪柜运输到位后多身份验证开启;民用方面:银行现金及贵重装备押运箱押运到位后多身份验证开启,大型租赁装备多身份验证使用限制等方面.该文提出了一种基于混沌映射公钥机制的多身份验证机制算法解决了以上多身份验证问题.目前在这方面,国内外相应的研究比较少.     

Velocity Modeling and Inversion Techniques for Locating Microseismic Events in Unconventional Reservoirs

A velocity model is an important factor influencing microseismic event locations. We re-view the velocity modeling and inversion techniques for locating microseismic events in exploration for unconvent...     

Structure and function of Zika virus NS5 protein: perspectives for drug design

Zika virus (ZIKV) belongs to the positive-sense single-stranded RNA-containing Flaviviridae family. Its recent outbreak and association with human diseases (e.g. neurological disorders) have raised global health concerns, and an urgency to develop a therapeutic strategy against ZIKV infection. However, there is no currently approved antiviral against ZIKV. Here we present a comprehensive overview on recent progress in structure–function investigation of ZIKV NS5 protein, the largest non-structural protein of ZIKV, which is responsible for replication of the viral genome, RNA capping and suppression of host interferon responses. Structural comparison of the N-terminal methyltransferase domain and C-terminal RNA-dependent RNA polymerase domain of ZIKV NS5 with their counterparts from related viruses provides mechanistic insights into ZIKV NS5-mediated RNA replication, and identifies residues critical for its enzymatic activities. Finally, a collection of recently identified small molecule inhibitors against ZIKV NS5 or its closely related flavivirus homologues are also discussed.     

Degradation of the mitochondrial complex I assembly factor TMEM126B under chronic hypoxia

Cell stress such as hypoxia elicits adaptive responses, also on the level of mitochondria, and in part is mediated by the hypoxia-inducible factor (HIF) 1α. Adaptation of mitochondria towards acute hypoxic conditions is reasonably well understood, while regulatory mechanisms, especially of respiratory chain assembly factors, under chronic hypoxia remains elusive. One of these assembly factors is transmembrane protein 126B (TMEM126B). This protein is part of the mitochondrial complex I assembly machinery. We identified changes in complex I abundance under chronic hypoxia, in association with impaired substrate-specific mitochondrial respiration. Complexome profiling of isolated mitochondria of the human leukemia monocytic cell line THP-1 revealed HIF-1α-dependent deficits in complex I assembly and mitochondrial complex I assembly complex (MCIA) abundance. Of all mitochondrial MCIA members, we proved a selective HIF-1-dependent decrease of TMEM126B under chronic hypoxia. Mechanistically, HIF-1α induces the E3-ubiquitin ligase F-box/WD repeat-containing protein 1A (β-TrCP1), which in turn facilitates the proteolytic degradation of TMEM126B. Attenuating a functional complex I assembly appears critical for cellular adaptation towards chronic hypoxia and is linked to destruction of the mitochondrial assembly factor TMEM126B.     

Junctional adhesion molecule-A: functional diversity through molecular promiscuity

Cell adhesion molecules (CAMs) of the immunoglobulin superfamily (IgSF) regulate important processes such as cell proliferation, differentiation and morphogenesis. This activity is primarily due to their ability to initiate intracellular signaling cascades at cell–cell contact sites. Junctional adhesion molecule-A (JAM-A) is an IgSF-CAM with a short cytoplasmic tail that has no catalytic activity. Nevertheless, JAM-A is involved in a variety of biological processes. The functional diversity of JAM-A resides to a large part in a C-terminal PDZ domain binding motif which directly interacts with nine different PDZ domain-containing proteins. The molecular promiscuity of its PDZ domain motif allows JAM-A to recruit protein scaffolds to specific sites of cell–cell adhesion and to assemble signaling complexes at those sites. Here, we review the molecular characteristics of JAM-A, including its dimerization, its interaction with scaffolding proteins, and the phosphorylation of its cytoplasmic domain, and we describe how these characteristics translate into diverse biological activities.